Tn5053 family transposons are res site hunters sensing plasmidal res sites occupied by cognate resolvases

North End Res

Res Judicata in California

Nucleic Acids Res

DNA microarrays enable users to obtain information on differences in transcript abundance on a massively parallel scale. Recently, however, data analyses have revealed potential pitfalls related to image acquisition, variability and misclassifications in replicate measurements, crosshybridization and sensitivity limitations. We have generated a series of analytical tools to address the manufact...

Site-specific relaxation and recombination by the Tn3 resolvase: recognition of the DNA path between oriented res sites.

We studied the dynamics of site-specific recombination by the resolvase encoded by the Escherichia coli transposon Tn3. The pure enzyme recombined supercoiled plasmids containing two directly repeated recombination sites, called res sites. Resolvase is the first strictly site-specific topoisomerase. It relaxed only plasmids containing directly repeated res sites; substrates with zero, one or tw...

RES 162 doc FINAL. qxp4